PDNA as building blocks for membrane-guided self-assemblies.

Content Provider	Semantic Scholar
Author	Pompon, Denis Laisné, Aude
Copyright Year	2007
Abstract	Different semi-synthetic PDNAs (protein-DNA complexes), which encompass a protein core engineered from the cytochrome b(5) scaffold, an embedded tuneable redox cofactor, a synthetic linker and a large oligonucleotide, were designed, synthesized and purified to homogeneity. These building blocks can be reversibly attached to Ni-DOGS [1,2-dioleoyl-sn-glycero-3-[N(5-amino-1-carboxypentyl)iminodiacetic acid]succinyl]-doped supported membranes through a metal chelate bridge with the protein part and be polymerized in a fully controllable manner using a solid-phase synthesis strategy and a stepwise addition of suitable complementary oligonucleotides. The resulting structures could recreate a large range of regular distribution of patterned redox and absorbing centres separated by fully tuneable distances and geometry. Kinetic parameters for the self-assembly of building blocks were determined using SPRI (surface plasmon resonance imagery). Structures of resulting nano-objects were characterized using gel electrophoresis and single molecule approaches following decoration of assemblies with quantum dots.
File Format	PDF HTM / HTML
DOI	10.1042/BST0350495
PubMed reference number	17511637
Journal	Medline
Volume Number	35
Part	3
Alternate Webpage(s)	http://www.biochemsoctrans.org/content/ppbiost/35/3/495.full.pdf
Alternate Webpage(s)	https://doi.org/10.1042/BST0350495
Journal	Biochemical Society transactions
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article