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An intragenic suppressor of the Arabidopsis floral organ identity mutant apetala3-1 functions by suppressing defects in splicing.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jack, Thomas |
| Copyright Year | 1998 |
| Abstract | The Arabidopsis floral organ identity gene APETALA3 (AP3) specifies the identity of petals and stamens in the flower. In flowers mutant for the temperature-sensitive ap3-1 allele, the petals and stamens are partially converted to sepals and carpels, respectively. ap3-1 contains a single nucleotide change in the AP3 gene that alters both an amino acid in the AP3 protein and the 5' splice consensus site for intron 5. Surprisingly, the Ap3-1 mutant phenotype is not due to the missense mutation but instead is due to defects in splicing; specifically, exon 5 is frequently skipped by the splicing machinery at the restrictive temperature. In a screen for suppressors of ap3-1, we isolated an intragenic suppressor, ap3-11, that functions to suppress the splicing defects of ap3-1. Using a reverse transcriptase-polymerase chain reaction assay, we demonstrate that the percentage of full-length exon 5-containing AP3 RNAs correlates with the phenotype of the flowers in both ap3-1 and ap3-11. Rather surprisingly, the ap3-11 suppressor mutation is located in intron 4. One model explaining the function of ap3-11 is that the ap3-11 suppressor creates a novel branch point sequence that causes exon 5 to be more frequently recognized by the splicing machinery. The identification of such a suppressor strongly suggests that exon-scanning models of intron-exon recognition are operative in plants. |
| Starting Page | 284 |
| Ending Page | 290 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.plantcell.org/content/plantcell/10/9/1465.full.pdf |
| PubMed reference number | 9724693v1 |
| Volume Number | 10 |
| Issue Number | 9 |
| Journal | The Plant cell |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Amino Acids Flowers Interferon Alfa-2b 10000000 UNT Injection [Intron A] Introns Missense Mutation Nucleotides RNA Splicing Reverse Transcriptase Polymerase Chain Reaction Suppressor Mutation |
| Content Type | Text |
| Resource Type | Article |
