NDLI: MicroRNA-204 regulates vascular smooth muscle cell calcification in vitro and in vivo.

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MicroRNA-204 regulates vascular smooth muscle cell calcification in vitro and in vivo.

Content Provider	Semantic Scholar
Author	Cui, Rong-rong Li, Shi-jun Liu, Ling-juan Yi, Lu Ying Liang, Qiu-hua Zhu, Xiao Chun Liu, Guan-Ying Liu, Yuan Wu, Shan-shan Liao, Xiao-Bo Yuan, Ling-Qing Mao, Ding-an Liao, Er-yuan
Copyright Year	2012
Abstract	AIMS Medial artery calcification is a common macroangiopathy that initiates from a cell-regulated process similar to osteogenesis. Although the mechanisms governing this process remain unclear, epigenomic regulation by specific microRNAs might play a role in vascular smooth muscle cell (VSMC) calcification. In this study, we aimed to investigate whether miR-204 participates in the regulation of VSMC calcification. METHODS AND RESULTS We found that miR-204 was suppressed in mouse aortic VSMCs during β-glycerophosphate-induced calcification, whereas Runx2 protein levels were elevated. Overexpression of miR-204 by transfection of miR-204 mimics decreased Runx2 protein levels and alleviated β-glycerophosphate-induced osteoblastic differentiation of VSMCs, whereas miR-204 inhibition by transfection of miR-204 inhibitors significantly elevated Runx2 protein levels and enhanced osteoblastic differentiation of VSMCs, suggesting the role of miR-204 as an endogenous attenuator of Runx2 in VSMC calcification. Luciferase reporter assays revealed Runx2 as the direct target of miR-204 by overexpression of miR-204 on the wild-type or mutant 3'-UTR sequences of Runx2 in VSMCs. In vivo overexpression of miR-204 by injection of miR-204 agomirs in Kunming mice attenuated vitamin D3-induced medial artery calcification. CONCLUSION Our study has shown that down-regulation of miR-204 may contribute to β-glycerophosphate-induced VSMC calcification through regulating Runx2. miR-204 represents an important new regulator of VSMC calcification and a potential therapeutic target in medial artery calcification.
Starting Page	323
Ending Page	329
Page Count	7
File Format	PDF HTM / HTML
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Alternate Webpage(s)	https://watermark.silverchair.com/cvs258.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAbMwggGvBgkqhkiG9w0BBwagggGgMIIBnAIBADCCAZUGCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM1f5C5jQIrqvDk4dTAgEQgIIBZr7y6mJTUHsOxywn88ZifnvTpFjd_HftkaBtUxS01G-ABQKfGZELOnqINPRA5N0yvGXxYjcCOokIx7-xpbodFj1ykFIy7_wKymNFZlBRkN-6zD-sDF6K69owPtmqiUNYlFZ0Q6os5uB_oWZIQR0UcFCRMo9YPknIDIBjncnOwVVivXS44loiCJWaVZm0QRiBgOjzIyfJ9omhGVgPUGCQctKSW-kmVPE5kXSmcenbrzKyk4JvWhPfm1llKAaa5LB6NtL8JJXJV2YXGe9jvUqsAEJRMzJdKAMcj3e3pnN4xkujdLujXUl4s55UNe0m0eKV6yK5e2oQGg3e7kSTPRHOTNdEq5QC7cIvmCUUfffau1diM_20woPYXAErxTedkg9oubzQtBhoSpabgjeLjOl9V6P6beEimAEYZese8bQiuJHCOgL5stN2KnRKLW_54KsRG3Dj-NbtRq9NtqHStEocPbWZRUI1Vwg
PubMed reference number	22871591v1
Alternate Webpage(s)	https://doi.org/10.1093/cvr/cvs258
DOI	10.1093/cvr/cvs258
Journal	Cardiovascular research
Volume Number	96
Issue Number	2
Language	English
Access Restriction	Open
Subject Keyword	Attenuator Device Component Calcinosis Down-Regulation Epigenomics Leukemia, B-Cell Luciferases MIRN204 microRNA, mouse Muscle Cells Muscle, Smooth, Vascular Myocytes, Smooth Muscle RUNX2 gene Smooth muscle (tissue) Vascular calcification Vascular smooth muscle cell
Content Type	Text
Resource Type	Article

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