NDLI: Colocalization of dopamine receptor subtypes with dopamine and cAMP-regulated phosphoprotein (DARPP-32) in rat brain

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Colocalization of dopamine receptor subtypes with dopamine and cAMP-regulated phosphoprotein (DARPP-32) in rat brain

Content Provider	Semantic Scholar
Author	Rajput, Padmesh S. Kharmate, Geetanjali Somvanshi, Rishi Kumar Kumar, Ujendra
Copyright Year	2009
Abstract	In the present study using indirect immunofluorescence immunohistochemistry, co-immunoprecipitation and western blot analysis we determined the colocalization of dopamine receptors 1-5 and dopamine and cAMP-regulated phosphoprotein (DARPP-32) in rat brain cortex and striatum. All five DR subtypes and DARPP-32 were expressed in rat brain cortex and striatum. DARPP-32 positive neurons displayed comparative colocalization with DR1-5. In cingulate cortex, the colocalization of DR subtypes was greatly different from frontal or temporal cortex. D1R is one of the most predominant subtypes which colocalized with DARPP-32 in cortex as well as striatum and followed by D2R, D3R, D4R and D5R. Amongst all DR subtypes D5R was coexpressed the least with DARPP-32 positive neurons. Consistent with immunohistochemical data, western blot analysis also reveals comparable distribution of DR subtypes and DARPP-32 in cortex and striatum. Colocalization studies were also supported by using co-immunoprecipitate assay displaying DARPP-32 expression in DR immunoprecipitate from tissue lysate prepared from cortex and striatum. Taken together our data support receptor specific association of DARPP-32 with DR subtypes that might shed new information in drugs of abuse and pathophysiology of neurodegenerative diseases as well as neuropsychiatric disorders such as schizophrenia.
Starting Page	53
Ending Page	63
Page Count	11
File Format	PDF HTM / HTML
DOI	10.1016/j.neures.2009.05.005
PubMed reference number	19465068
Journal	Medline
Volume Number	65
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S0168010209001710
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S0168010209001710?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/j.neures.2009.05.005
Journal	Neuroscience Research
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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