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UvA-DARE ( Digital Academic Repository ) Thrombopotein : its ups and downs
| Content Provider | Semantic Scholar |
|---|---|
| Author | Folman, Claudia C. Link |
| Copyright Year | 2011 |
| Abstract | Thrombopoietin is produced at a constant rate by the liver and kidney and is removed from the circulation upon binding and subsequent uptake via the Tpo receptor, c-Mpl, expressed by platelets and megakaryocytes. Apart from uptake, this study shows that platelets can also function as a storage pool for Tpo. Upon stimulation with various platelet agonists, full-length biologically active Tpo was released by platelets. Platelet fractionation experiments indicated that this Tpo is contained in the granules. When platelets were preincubated with Tpo-peptide mimetic or truncated Tpo prior to activation, a threeto fivefold increment in Tpo release was seen. However, no enhanced release of other granule proteins such as vWF-propeptide or serotonin occurred. Therefore, the Mpl agonists might compete with Mpl-bound Tpo, thereby releasing Tpo into the platelet supernatant. Intravascular release of Tpo by platelets might occur in patients with massive platelet activation, as occurs in patients with disseminated intravascular coagulation. The Tpo concentration in these patients is elevated (p<0.01) and correlates with markers for thrombin generation, TAT complexes and Fl+2 (rp=0.8 and 0.9; pO.01). This suggests that the increment in Tpo concentration may be attributed to Tpo release by activated platelets in vivo, which might be instrumental in subsequent stimulation of thrombocytopoiesis. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/3194840/14815_A01_061_003.pdf |
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| Alternate Webpage(s) | https://pure.uva.nl/ws/files/3194838/14814_A01_061_002.pdf |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/3194842/14816_A01_061_004.pdf |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/3194834/14812_A01_061.pdf |
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| Alternate Webpage(s) | https://pure.uva.nl/ws/files/3194868/14829_A01_061_017.pdf |
| Alternate Webpage(s) | https://pure.uva.nl/ws/files/3194866/14828_A01_061_016.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
