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Effect of acemannan, an extracted polysaccharide from Aloe vera, on BMSCs proliferation, differentiation, extracellular matrix synthesis, mineralization, and bone formation in a tooth extraction model
| Content Provider | Semantic Scholar |
|---|---|
| Author | Boonyagul, Sani Banlunara, Wijit Sangvanich, Polkit Thunyakitpisal, Pasutha |
| Copyright Year | 2012 |
| Abstract | Aloe vera is a traditional wound healing medicine. We hypothesized acemannan, a polysaccharide extracted from Aloe vera gel, could affect bone formation. Primary rat bone marrow stromal cells (BMSCs) were treated with various concentrations of acemannan. New DNA synthesis, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein, osteopontin expression, and mineralization were determined by [3H] thymidine incorporation assay, ELISA, biochemical assay, western blotting, and Alizarin Red staining, respectively. In an animal study, mandibular right incisors of male Sprague–Dawley rats were extracted and an acemannan treated sponge was placed in the socket. After 1, 2, and 4 weeks, the mandibles were dissected. Bone formation was evaluated by dual-energy X-ray absorptiometry and histopathological examination. The in vitro results revealed acemannan significantly increased BMSC proliferation, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein and osteopontin expression, and mineralization. In-vivo results showed acemannan-treated groups had higher bone mineral density and faster bone healing compared with untreated controls. A substantial ingrowth of bone trabeculae was observed in acemannan-treated groups. These data suggest acemannan could function as a bioactive molecule inducing bone formation by stimulating BMSCs proliferation, differentiation into osteoblasts, and extracellular matrix synthesis. Acemannan could be a candidate natural biomaterial for bone regeneration. |
| Starting Page | 310 |
| Ending Page | 317 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s10266-012-0101-2 |
| PubMed reference number | 23315202 |
| Journal | Medline |
| Volume Number | 102 |
| Alternate Webpage(s) | http://www.desertharvest.com/physicians/documents/Acemannan1.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/s10266-012-0101-2 |
| Journal | Odontology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
