NDLI: Formes soluble et membranaire de la choline acétyltransférase chez la mouche "Drosophila melanogaster"

Content Provider	Semantic Scholar
Author	Salem, Natalie
Copyright Year	1994
Abstract	La choline acethyltransferase (ChAT) catalyse la biosynthese du neurotransmetteur acetylcholine. Outre sa forme soluble, hydrophile, nous avons demontre l'existence d'une forme membranaire, amphiphile, de la ChAT dans la tete de Drosophile. Les taux d'enzyme membranaire augmentent au cours du developpement. Nous avons clone un ADNc complet (4.2 kb) codant pour la ChAT de Drosophile. Les ovocytes de Xenope injectes expriment une activite amphiphile. L'activite amphiphile n'est pas enrichie dans les membranes. La ChAT amphiphile native et recombinante sont moins sensibles a l'inhibition par l'acetylcholine ainsi qu'a la denaturation thermique. Des fibroblastes et des neuroblastomes transfectes de facon stable expriment une activite amphiphile. Il semble que la ChAT amphiphile ne soit enrichie que dans les membranes de cellules d'origine nerveuse. Nous avons produit un anticorps contre une proteine de fusion couvrant la partie C-terminale de la ChAT. Cet anticorps reconnait la proteine precurseur de l'enzyme de Drosophile a 75 kDa.
File Format	PDF HTM / HTML
DOI	10.13097/archive-ouverte/unige:104643
Alternate Webpage(s)	https://archive-ouverte.unige.ch/files/downloads/0/0/1/0/4/6/4/3/unige_104643_attachment01.pdf
Alternate Webpage(s)	https://doi.org/10.13097/archive-ouverte%2Funige%3A104643
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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