NDLI: A New Continuous Cell Line of Spodoptera exigua and Its Susceptibility to Autographa californica Multicapsid Nucleopolyhedrovirus

Please wait, while we are loading the content...

A New Continuous Cell Line of Spodoptera exigua and Its Susceptibility to Autographa californica Multicapsid Nucleopolyhedrovirus

Content Provider	Semantic Scholar
Author	Chaeychomsri, Sudawan Chaeychomsri, Win Ikeda, Motoko Kobayashi, Michihiro
Copyright Year	2016
Abstract	A continuous cell line, designated KU-SENL-1 (SENL1), has been established from minced neonate larvae of Spodoptera exigua (Lepidoptera: Noctuidae) treated with collagenase. The primary culture was maintained in TC100 medium supplemented with 10% Fetal Bovine Serum (FBS), 3% Helicoverpa armigera hemolymph and incubated at 27°C. This continuous cell line was cultured in TC100 medium supplemented with 10% FBS and subcultured at 5day intervals. The cell line consisted of a mixture of two cell types, epithelial-like cells and spindle-shaped cells, both of which grown as attached monolayers. The population doubling time of this new cell line during the logarithmic phase of growth was 45h. RAPD and DAF analyses confirmed that the origination of the SENL1 cell line was S. exigua. The susceptibility of this cell line to the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) was high and by 3 days postinfection (pi) greater than 90% of the cells contained Occlusion Bodies (OBs) or were greatly hypertrophied, indicating they were infected. This cell line was highly effective for Budded Viruses (BV) titration of the AcMNPV. Therefore, the SENL1 cell line will be a valuable new tool for biological characterization of AcMNPV in cell culture and also for protein expression using the baculovirus-insect cell expression vector system.
Starting Page	231
Ending Page	238
Page Count	8
File Format	PDF HTM / HTML
DOI	10.18178/joaat.3.4.231-238
Volume Number	3
Alternate Webpage(s)	http://www.joaat.com/uploadfile/2016/1230/20161230104640798.pdf
Alternate Webpage(s)	https://doi.org/10.18178/joaat.3.4.231-238
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in