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PENGARUH KINETIN TERHADAP INISIASI DAN PERTUMBUHAN TUNAS PADA PERBANYAKAN TANAMAN JARAK PAGAR (Jatropha curcas L.) SECARA IN VITRO
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dewi, Prita Sari Susanti, Dyah Ochtorina |
| Copyright Year | 2010 |
| Abstract | Perbanyakan in vitro jarak pagar (Jatropha curcas L.) diupayakan sebagai langkah alternatif dalam perbanyakan bibit untuk mendukung perbanyakannya secara konvensional yang terkendala oleh masih belum tersedianya materi dengan potensi genetik yang seragam dalam jumlah yang memadai. Penelitian bertujuan mengetahui pengaruh penambahan kinetin dan NAA pada media Murashige and Skoog (MS) dalam perbanyakan in vitro jarak pagar beserta konsentrasi yang terbaik untuk mendapatkan hasil yang optimal. Percobaan disusun dalam Rancangan Acak Kelompok Lengkap (RAKL) dengan tiga kali ulangan. Faktor yang dicoba adalah 7 taraf konsentrasi kinetin. Variabel yang diamati meliputi kedinian terbentuknya tunas, jumlah tunas per eksplan, tinggi tunas. Hasil penelitian menunjukkan bahwa pemberian kinetin lebih dari 1,00 ppm dapat meningkatkan pertumbuhan tunas, terutama pada konsentrasi 2,00 ppm. Kata kunci: jarak pagar (Jatropha curcas L), perbanyakan in vitro, kinetin ABSTRACT In vitro propagation of physic nut (Jatropha curcas L.) was undertaken as an alternative multiplication mean of its planting material to support conventional propagation method which have been hampered due to lack of low potential genetic material in a sufficient number. The study aimed to investigate the effect of kinetin with its optimal concentration in Murashige and Skoog (MS) media on in vitro propagation of physic nut. The experiment was conducted using Completely Randomized Block Design (RCBD) with three replications. The tested factors were seven levels of kinetin concentration. The observed variables were induction time of shoot, number of shoots per explant and height of shoot. The result showed that addition of kinetin more than 1.00 ppm can increase growth of shoot especially on concentration 2.00 ppm. Key words: physic nut (Jatropha curcas L.), In vitro propagation, kinetin |
| File Format | PDF HTM / HTML |
| DOI | 10.20884/1.agrin.2010.14.1.98 |
| Alternate Webpage(s) | https://jurnalagrin.net/index.php/agrin/article/download/98/84 |
| Alternate Webpage(s) | https://doi.org/10.20884/1.agrin.2010.14.1.98 |
| Volume Number | 14 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |