NDLI: Change in local dynamics of bacteriorhodopsin with retinal isomerization under pressure as studied by fast magic angle spinning NMR

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Change in local dynamics of bacteriorhodopsin with retinal isomerization under pressure as studied by fast magic angle spinning NMR

Content Provider	Semantic Scholar
Author	Kawamura, Izuru Yamaguchi, Satoru Nishikawa, Hirohide Tajima, Kana Horigome, Miyako Tuzi, Satoru Saitô, Hazime Naito, Akira
Copyright Year	2012
Abstract	Bacteriorhodopsin (bR) has a retinal with all-trans and 13-cis, 15-syn configurations whose isomeric ratio is close to 1 in the dark-adapted bR, and the population of the 13-cis, 15-syn configuration can be increased under pressure. In our previous study, we applied pressure to bR using centrifugal force introduced by sample spinning and demonstrated that the 15N nuclear magnetic resonance (NMR) signal intensity of the Schiff base in 13-cis, 15-syn retinal increased. In this study, we demonstrate a pressure-induced change in the local dynamics of the protein side in the vicinity of retinal. In the 13C dipolar decoupling and magic angle-spinning NMR spectra of [3-13C]Ala-labeled bR under fast spinning at 10 and 12 kHz, corresponding to 44 and 63 bar, respectively, the 13C NMR signal at 16.6 p.p.m. appeared prominently as a sharp peak. This peak is assigned to Ala81 and Ala84 residues located in the vicinity of the retinal. It was observed that the change in the local dynamics in the vicinity of the retinal was caused by the applied pressure. These experiments demonstrate that fast magic angle spinning in NMR provides a pressure source for investigating the structure and change in the dynamics of biomacromolecules. Polymer Journal (2012) 44, 863–867; doi:10.1038/pj.2012.116; published online 13 June 2012
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.nature.com/pj/journal/v44/n8/pdf/pj2012116a.pdf
Language	English
Access Restriction	Open
Subject Keyword	Bacteriorhodopsins Bromides Centrifugal governor Coupling (computer programming) Experiment Kilohertz Magnetic Resonance Imaging PersonNameUse - assigned Polymer Retina Retinaldehyde Schiff Bases Scientific Publication Source-to-source compiler Tracer Virtual retinal display
Content Type	Text
Resource Type	Article

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