NDLI: Genetic Similarity within Isolates of Population of the Cattle Tick, Rhipicephalus microplus Based on Mitochondrial Cytochrome Oxidase Subunit-I Gene Sequences

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Genetic Similarity within Isolates of Population of the Cattle Tick, Rhipicephalus microplus Based on Mitochondrial Cytochrome Oxidase Subunit-I Gene Sequences

Content Provider	Semantic Scholar
Author	Veeramani, Velayutham Sakthivelkumar, Shanmugavel Tamilarasan, Krishnamurthi Janarthanan, Sundaram
Copyright Year	2014
Abstract	Molecular phylogenetic analysis was carried out using mitochondrial nucleotide sequences (COI) to provide evidence in genetic relatedness among various isolates of cattle tick Rhipicephalus microplus collected from different locations in Chennai, Tamil Nadu, India. The mitochondrial COI gene region of ticks was amplified and sequenced. A multiple sequence alignment using edited sequences of various isolates of ticks was carried out and a phylogenetic tree was constructed. The results on multiple sequence alignment and phylogram revealed that COI gene sequence of the eight isolates of ticks collected had more than 99.90 % similarity. It confirmed that COI gene was most conserved in the mitochondrial DNA of the collected cattle tick, R. microplus. Subsequently, the sequences were used to predict the secondary structure models of COI which also revealed similar such close relationships. These results in the present study reflect a single monophyletic group with no functional diversity at the mitochondrial COI gene region either in the sequence or structures was observed at various isolates of cattle tick R. microplus. The indication of absence of genetic variation within isolates of ticks was revealed in the present.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.idosi.org/gv/gv13(2)14/6.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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