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Evidence for different patterns of post-translational processing of Pro-enkephalin in the bovine adrenal, colon and striatum indicated by radioimmunoassay using region-specific antisera to Met-Enk-Arg6-Phe7 and Met-Enk-Arg6-Gly7-Leu8
| Content Provider | Semantic Scholar |
|---|---|
| Author | Giraud, Andrew S. Williams, Richard G. Dockray, Graham J. |
| Copyright Year | 1984 |
| Abstract | Specific antisera to two opioid peptides, Met-Enk-Arg6-Phe7 and Met-Enk-Arg6-Gly7-Leu8, were used in radioimmunoassay to study molecular forms in extracts of bovine adrenal, striatum and colon. The antisera to Met-Enk-Arg6-Phe7 and Met-Enk-Arg6-Gly7-Leu8 were each highly specific for the C-terminus of their respective haptens and showed less than 1% cross-reactivity with related enkephalins and their variants. In adrenal extracts, gel filtration on Sephadex G-50 indicated N-terminally extended forms of Met-Enk-Arg6-Phe7 and Met-Enk-Arg6-Gly7-Leu8 as the predominating immunoreactive species, whereas in the striatum and colon the main immunoreactive forms were compatible with the hepta- and octapeptides. The results suggest different patterns of post-translational processing of precursors in the adrenal medulla compared with striatal and colonic neurones. |
| Starting Page | 223 |
| Ending Page | 228 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0304-3940(84)90446-4 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/0304394084904464 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/0304394084904464?dgcid=api_sd_search-api-endpoint |
| PubMed reference number | 6738916 |
| Alternate Webpage(s) | https://doi.org/10.1016/0304-3940%2884%2990446-4 |
| Journal | Medline |
| Volume Number | 46 |
| Journal | Neuroscience Letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
