The effect of IL-28 A on human cord blood CD 4 + T cells

Content Provider	Semantic Scholar
Author	Arasteh, Javad Ebtekar, Masoumeh Pourpak, Zahra Pourfatollah, Ali Akbar Hassan, Zuhair Mohammad Farahmandian, Tahereh
Copyright Year	2010
Abstract	Background and aim: The utilization of umbilical cord blood transplantation (UCBT) has been increasing because of the potential advantage of rapid accessibility and the lesser risk of graft-versus-host disease (GVHD), thus allowing less strict HLA matching. IL-28A, also known as IFN-λ2, has been regarded as a member of a new cytokine family that shares some features with type I interferon (IFN) and was shown to have antiviral activity. The aim of this study was to identify biological activity of IL-28 on cord blood CD4+ T cells. Materials and methods: In this study, we cultured CD4+ T cells with IL-28A (20 ng/ml), IL-2 (20 ng/ml) and 5μg/ml MACS Anti-Biotin MACSiBead Particles (bead-to-cell ratio 1:2) for 2 weeks. Results: Flow cytometry analyses showed that IL-28A cannot be effective on CD25 and Foxp3 expression on cord blood CD4+ T cells, and it is not involved in proliferation of these cells. Treg suppression assay also showed that this cytokine cannot induce production of regulatory T cells. Conclusion: We showed that IL-28A is not involved in expression of CD25 and Foxp3 markers and proliferation of CD4+CD25− T cells, and that our findings also suggest that induction of Foxp3 in T cells activated by anti-CD3/anti-CD28 does not result in the regulatory activity in these cells.
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Language	English
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Content Type	Text
Resource Type	Article