NDLI: Deteksi Mycobacterium tuberculosis dan resistensinya terhadap rifampisin dengan Metode nested polymerase chain reaction (PCR) dan sequencing

Please wait, while we are loading the content...

Deteksi Mycobacterium tuberculosis dan resistensinya terhadap rifampisin dengan Metode nested polymerase chain reaction (PCR) dan sequencing

Content Provider	Semantic Scholar
Author	Rosilawati, Maria Lina
Copyright Year	2016
Abstract	Latar Belakang DNA rpob (RNA polymerase sub unit b) Mycobacterium tuberculosis dapat diamplifikasi secara spesifik dengan metode nested polymerase chain reaction (PCR). Nested PCR yang dilanjutkan dengan sequencing dapat secara langsung diaplikasikan untuk mendeteksi M. tuberculosis dan menentukan mutasi pada gen rpob yang berkaitan dengan resistensinya terhadap rifampisin, pada isolat klinis maupun sampel sputum. Metode Dalam penelitian ini digunakan 20 isolat klinis dan 30 sampel sputum yang diamplifikasi dengan primer yang dirancang dari bagian gen rpob M. tuberculosis. Metode fenol-kloroform dan metode Boom masing-masing digunakan untuk ekstraksi DNA isolat klinis dan sampel sputum. Sequencing hanya dilakukan untuk hasil PCR dari sampel sputum. Hasil Dari 20 isolat klinis, 15 isolat positif terdeteksi sebagai M. tuberculosis dengan nested PCR, 4 isolat tergolong Mycobacteria other than tuberculosis (MOTT) dan 1 isolat non-Mycobacteria. Hasil nested PCR pada 30 sampel sputum dengan 25 sampel basil tahan asam (BTA) positif dan 5 sampel BTA negatif, menunjukkan hasil positif pada 21 sampel. Besarnya produk first-round dan second-round PCR masing-masing adalah 205 bp dan 157 bp. Kesimpulan Nested PCR dengan sequencing lebih sensitif dan spesifik dalam mendeteksi M. tuberculosis dan resistensinya terhadap rifampisin.
Starting Page	1
Ending Page	10
Page Count	10
File Format	PDF HTM / HTML
DOI	10.18051/UnivMed.2007.v26.1-10
Volume Number	26
Alternate Webpage(s)	https://univmed.org/ejurnal/index.php/medicina/article/download/290/243
Alternate Webpage(s)	https://doi.org/10.18051/UnivMed.2007.v26.1-10
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in