Production of callus mediated gynogenic haploids in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.)

Content Provider	Semantic Scholar
Author	Kurtar, Ertan Sait Balkaya, Ahmet Ozer, Mete
Copyright Year	2017
Abstract	Kurtar E.S., Balkaya A., Ozbakir Ozer M. (2018): Production of callus mediated gynogenic haploids in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.). Czech J. Genet. Plant Breed., 54: 9−16. Although haploids were successfully produced via irradiated pollen technique and anther culture in Cucurbita maxima and Cucurbita moschata, the haploidization efficiency is still low due to genotype dependence. Thus, as an alternative technique, the efficacy of the ovule culture was investigated. Ovules were extracted at different flowering time and then cultured on a solid MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP), thidiazuron (TDZ), and naphthaleneacetic acid (NAA) to induce callogenesis and plant regeneration. The gynogenic response was influenced by the combination of plant growth regulators, genotype and culture time. The medium containing of 4.0 mg/l BAP + 0.05 mg/l NAA + 0.1 mg/l TDZ provided the highest response at anthesis time. Plantlets were rooted and elongated on a solid MS medium supplemented with 0.01 mg/l indole-3-acetic acid (IAA) + 1.0 mg/l BAP. The ploidy observations of 122 plants revealed that 70 plants were haploid, 46 plants were diploid and the others were mixoploid.
Starting Page	9
Ending Page	16
Page Count	8
File Format	PDF HTM / HTML
DOI	10.17221/30/2017-CJGPB
Volume Number	54
Alternate Webpage(s)	https://www.agriculturejournals.cz/publicFiles/243420.pdf
Alternate Webpage(s)	https://doi.org/10.17221/30%2F2017-CJGPB
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article