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Charakterisierung von Autoantikörpern gegen Protease-aktivierte Rezeptoren 1 und 2 und gegen Endothelin-Rezeptor ET(A)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Freier, J. |
| Copyright Year | 2008 |
| Abstract | Some patients with Raynaud's syndrome, urticaria, coronary artery disease, Angina or pulmonary hypertension have functional autoantibodies against thrombin receptors PAR1/2 and/or against endothelin receptor ET(A). In this work the effects of such patients' IgG preparations on functions of ventricular cardiomyocytes of neonatal rats, human smooth muscle cells from coronary arteries (hCASMC); freshly isolated, human platelets as well as monocytes were investigated. For comparison, the PAR agonists thrombin and the stimulating peptide SFLLRN as well as endothelin-1 were used. While purified autoantibodies against ET(A) did not activate ERK1/2 in cardiomyocytes, IgG preparations with autoantibodies against PAR1/2 resulted in a similar activation as the peptide SFLLRN. Surprisingly, control IgG also caused a strong activation of ERK1/2. Coincubation of cardiomyocytes with antibody preparations and IL-1beta increased the phosphorylation of ERK1/2 in all cases. In hCASMCs, IgG preparations with PAR-autoantibodies and control IgGs caused activation of ERK1/2, whereas ET(A)-autoantibodies did not. The results of platelet activation with patients' IgG were varying. Tests with prestimulated platelets showed, that a stimulating effect of the autoantibodies on preactivated platelets can not be excluded. However, without prestimulation patients' IgG rather seemed to have an inhibiting effect on platelet function. Preincubation of monocytes with patients' IgG had no influence on PMA-induced production of superoxide anion compared with control IgG. Only two of five patients' IgGs showed a stimulating effect on monocytic ERK1/2 phosphorylation. In conclusion, PAR1/2-and ET(A)-autoantibodies showed no common effects on the function of vascular smooth muscle cells, platelets and monocytes. The differentiation of autoantibody-positive and-negative preparations of IgG only was possible by determining the beating rate of cardiomyocytes. autoantibody thrombin endothelin-1 cardiovascular PAR ET(A) |
| File Format | PDF HTM / HTML |
| DOI | 10.18452/15722 |
| Alternate Webpage(s) | http://edoc.hu-berlin.de/dissertationen/freier-jeannette-2007-12-13/PDF/freier.pdf |
| Alternate Webpage(s) | https://edoc.hu-berlin.de/bitstream/handle/18452/16374/freier.pdf?isAllowed=y&sequence=1 |
| Alternate Webpage(s) | https://doi.org/10.18452/15722 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
