Isolation and transplantation of dopaminergic neurons generated from mouse embryonic stem cells

Content Provider	Semantic Scholar
Author	Yoshizaki, Takahito Inaji, Motoki Kouike, Hiroko Shimazaki, Takuya Okano, Hideyuki
Copyright Year	2004
Abstract	Embryonic stem (ES) cells differentiate into dopamine (DA)-producing neurons when co-cultured with PA6 stromal cells, but the resulting cultures contain a variety of unidentified cells. In order to label live DA neurons in mixed populations, we introduced a GFP reporter under the control of the tyrosine hydroxylase (TH) gene promoter into ES cells. GFP expression was observed in TH-immunoreactive cells that differentiated from the ES cells that carried the TH-GFP reporter gene. DA neurons expressing GFP were sorted from the mixed cell population by fluorescence-activated cell sorting of cells exhibiting GFP fluorescence, and the sorted GFP(+) cells obtained were transplanted into a rat model of Parkinson's disease. Some of these cells survived and innervated the host striatum, resulting in a partial recovery from parkinsonian behavioral defects. This strategy of isolation and transplantation of ES-cell-derived DA neurons should be useful for cellular and molecular studies of DA neurons and for clinical application in the treatment of Parkinson's disease.
Starting Page	33
Ending Page	37
Page Count	5
File Format	PDF HTM / HTML
DOI	10.1016/j.neulet.2004.03.074
PubMed reference number	15157991
Journal	Medline
Volume Number	363
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S0304394004003660
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S0304394004003660?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/j.neulet.2004.03.074
Journal	Neuroscience Letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article