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Cloning and DNA sequencing of cytosolic Cu/Zn superoxide dismutase gene from chinese cabbage
| Content Provider | Semantic Scholar |
|---|---|
| Author | Keumna, Yoon Kim, Hyunuk Lee, Woosung |
| Copyright Year | 2009 |
| Abstract | A cDNA clone for the cytosolic Cu/Zn superoxide dismutase (Cu/Zn SOD) from Chinese cabbage (Brassica campestris ssp.pekinensis) was isolated and its DNA sequence was determined. The cDNA clone contains a complete coding sequence which encodes a protein of 152 amino acids and a 3-untranslated region including a poly A signal. The deduced amino acid sequence shows that it is highly homologous to the Cu/Zn SODs from other plants (60–90%). The lack of a putative chloroplast targeting transit peptide indicates that the clone represents a cytosolic form of Cu/Zn SOD. Genomic Southern hybridization suggests that cytosolic Cu/Zn SOD genes are present in 1 or 2 copies per genome. |
| Starting Page | 68 |
| Ending Page | 71 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/BF03030476 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/BF03030476 |
| Alternate Webpage(s) | https://doi.org/10.1007/BF03030476 |
| Volume Number | 41 |
| Journal | Journal of Plant Biology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |