Dithiothreitol Inhibits the Amyloid Fibril Formation of β-Lactoglobulin

Content Provider	Semantic Scholar
Author	Ma, Baoliang Wei, Liangshu Lu, Li-Ping Wu, Fang Ying
Copyright Year	2014
Abstract	Amyloid fibrils are associated with Alzheimer’s disease, Parkinson’s disease, and non-neuropathic systemic amyloidosis. The effect of DTT on the amyloid formation of β-lactoglobulin has been examined by thioflavin T binding, Congo red binding and transmission electron microscopy. The results showed that the formation of the amyloid fibril took place when β-lactoglobulin was incubated in the presence of 5M urea and at neural pH. However, the formation of amyloid fibrils was inhibited when DTT was added into the solutions and the inhibitory effect is dependent on the concentrations of DTT. These results demonstrate that the disulfide bonds play important roles in the formation of amyloid fibrils.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.hanspub.org/journal/PaperDownload.aspx?DOI=10.12677/BIPHY.2014.24005
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article