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High-resolution Imaging of Reconstituted Protein-DNA Complexes Using Phase Plate Electron Cryo Microscopy
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sandin, Sara Danev, Radostin Plitzko, Jürgen M. Vogirala, Vinod Kumar Wong, Andrew K. C. |
| Copyright Year | 2016 |
| Abstract | To understand mechanisms involved in gene silencing and activation in higher eukaryotes, detailed knowledge of the structural organization of protein-DNA complexes is needed. Nucleosomes represent the first level of DNA organization in eukaryotes. The Nucleosome Core Particle (NCP) is a 200kDa disk-shaped object, 110x57Å in diameter, composed of 50% protein and 50% DNA. Crystal structures of the NCP show that DNA is wrapped around a protein core, called the histone octamer, in 1.65 superhelical turns [1]. Different histone variants, post-translation modifications of histone proteins, DNA sequences and interacting proteins may directly or indirectly affect chromatin higher-order structure and recruitment of chromatin binding factors [1]. These factors and pathways are misregulated in many different human diseases, such as cancers and viral infections. |
| Starting Page | 68 |
| Ending Page | 69 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| DOI | 10.1017/s1431927616001197 |
| Alternate Webpage(s) | https://www.cambridge.org/core/services/aop-cambridge-core/content/view/3489803ADB3958960CF5839D5A50E0FE/S1431927616001197a.pdf/div-class-title-high-resolution-imaging-of-reconstituted-protein-dna-complexes-using-phase-plate-electron-cryo-microscopy-div.pdf |
| Alternate Webpage(s) | http://journals.cambridge.org/fulltext_content/supplementary/MAM22_S3_minisite/7337/0068.pdf |
| Alternate Webpage(s) | https://www.cambridge.org/core/services/aop-cambridge-core/content/view/3489803ADB3958960CF5839D5A50E0FE/S1431927616001197a.pdf/highresolution_imaging_of_reconstituted_proteindna_complexes_using_phase_plate_electron_cryo_microscopy.pdf |
| Alternate Webpage(s) | https://doi.org/10.1017/s1431927616001197 |
| Volume Number | 22 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
