Inhibition of Vancomycin-Resistant Enterococcus by Continuous-Flow Cultures of Human Stool Microflora With and Without Anaerobic Gas Supplementation

Content Provider	Semantic Scholar
Author	Hume, Michael Edwin Poole, T. L. Pultz, Nicole J. Hanrahan, Jennifer A. Donskey, Curtis J.
Copyright Year	2003
Abstract	A continuous-flow competitive exclusion (CFCE) culture model of human stool microflora was used to examine whether supplemental anaerobic gas is necessary for maintenance of anaerobes and inhibition of vancomycin-resistant Enterococcus (VRE). CFCE cultures of human stool microflora were maintained with supplemental nitrogen, without supplemental nitrogen, or with percolated room air. Cultures with or without supplemental nitrogen maintained >9 log10 CFU mL−1 of obligate anaerobes and eliminated 106 CFU mL−1 of VRE. When room air was percolated into the culture, anaerobes were detected at ≤2 log10 CFU mL−1, and the same VRE inoculum was not eliminated (P < 0.001). These data demonstrate that human stool CFCE cultures maintain high levels of obligate anaerobes and inhibit VRE without the addition of supplemental anaerobic gas.
Starting Page	364
Ending Page	367
Page Count	4
File Format	PDF HTM / HTML
DOI	10.1007/s00284-003-4112-7
PubMed reference number	15060733
Journal	Medline
Volume Number	48
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1007/s00284-003-4112-7
Alternate Webpage(s)	https://doi.org/10.1007/s00284-003-4112-7
Journal	Current Microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article