Neurons produce a neuronal cell surface-associated chondroitin sulfate proteoglycan.

Content Provider	Semantic Scholar
Author	Lander, Cynthia Zhang, Honglai Hockfield, Susan
Copyright Year	1998
Abstract	Monoclonal antibody Cat-315 recognizes a chondroitin sulfate proteoglycan (CSPG) expressed on the surface of subsets of neurons in many areas of the mammalian CNS (). The cell type-specific expression exhibited by the Cat-315 CSPG and other perineuronal net CSPGs imparts a distinct molecular surface identity to a neuron (Celio and Blumcke, 1994; Lander et al., 1997). The cell type(s) producing these surface-associated proteins and yielding this cellular diversity has remained in question. The expression of the Cat-315 CSPG in primary rat cortical cultures has permitted an examination of the cellular source of the Cat-315 antigen, as well as a determination of its spatial relationship to the neuronal surface. Live-cell labeling of primary neuronal cultures demonstrates that the Cat-315 CSPG is on the extracellular surface of neurons. Furthermore, extraction experiments demonstrate that the Cat-315 CSPG lacks a transmembrane domain and that the entire molecule is extracellular and, therefore, can be considered a constituent of brain extracellular matrix. Several lines of evidence indicate that neurons with cell surface staining produce the Cat-315 CSPG. First, neurons with cell surface staining also show intracellular Cat-315 immunoreactivity. Second, beta-xyloside or monensin, reagents that inhibit the synthesis and transport of CSPGs, increase intracellular Cat-315 immunoreactivity within neurons that express cell surface Cat-315 immunoreactivity. Third, double labeling with Cat-315 and a polyclonal antibody for the Golgi complex demonstrates a precise colocalization of the intracellular Cat-315 immunoreactivity with the Golgi. Together, these observations demonstrate that neurons contribute to the extracellular matrix of brain and that the Cat-315 CSPG is produced by the neurons that carry Cat-315 cell surface immunoreactivity.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.jneurosci.org/content/18/1/174.full.pdf
Alternate Webpage(s)	http://www.jneurosci.org/content/jneuro/18/1/174.full.pdf
PubMed reference number	9412498v1
Volume Number	18
Issue Number	1
Journal	The Journal of neuroscience : the official journal of the Society for Neuroscience
Language	English
Access Restriction	Open
Subject Keyword	Adrenergic beta-Antagonists Chondroitin Sulfate Proteoglycans Chondroitin Sulfates Extracellular Matrix Mammals Monensin Neurons Proteoglycan Reagents Transmembrane Domain perineuronal net (cell component) polyclonal antibody
Content Type	Text
Resource Type	Article