NDLI: Biofuel cell based on glucose oxidase from Penicillium funiculosum 46.1 and horseradish peroxidase

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Biofuel cell based on glucose oxidase from Penicillium funiculosum 46.1 and horseradish peroxidase

Content Provider	Semantic Scholar
Author	Ramanavicius, Arunas Kausaite-Minkstimiene, Asta Morkvėnaitė-Vilkončienė, Inga Genys, Povilas Mikhailova, Raisa Semashko, Tatiana A. Voronovic, Jaroslav Ramanaviciene, Almira
Copyright Year	2015
Abstract	Abstract Biofuel cells are bio-electrochemical devices, which are suitable for environmentally friendly generation of energy. Enzymatic biofuel cell (E-BFC) operating at ambient temperature and in low fuel concentrations is reported in this research. Glucose oxidase (GOx) from Penicillium funiculosum 46.1 and insoluble redox mediator 9,10-phenantroline-5,6-dione (PD) immobilized on graphite rod electrode (GRE) were applied in the design of biofuel cell anode (GOx/PD/GRE). Horseradish peroxidase (HRP) modified GRE was applied in cathode (HRP/GRE) of the same biofuel cell. GOx/PD/GRE anode demonstrated sufficient electro-catalytic activity during the oxidation of glucose, and HRP/GRE cathode exhibited direct electron transfer based electro-catalytic reduction of hydrogen peroxide. Both anode and cathode were operating in buffer, pH 6.0, consisting of 0.05 mol L −1 sodium acetate–sodium phosphate and 0.1 mol L −1 KCl. The maximal registered open-circuit-voltage of the complete E-BFC exceeded 640 mV, calculated maximal power density was 4.2 μW cm −2 at the cell voltage of 530 mV in the presence of 200 mmol L −1 or higher glucose and 0.1–0.5 mmol L −1 of hydrogen peroxide concentration in anode and cathode compartment, respectively. Such new design of biofuel cell allows to utilize glucose as a fuel for the anode, and H 2 O 2 formed during the catalytic action of GOx, which is immobilized on anode, could be utilized as a fuel at the cathode of the same biofuel cell.
Starting Page	165
Ending Page	173
Page Count	9
File Format	PDF HTM / HTML
DOI	10.1016/j.cej.2014.11.011
Volume Number	264
Alternate Webpage(s)	http://biochem.flas.kps.ku.ac.th/02738451/biofuel.pdf
Alternate Webpage(s)	https://doi.org/10.1016/j.cej.2014.11.011
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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