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Catabolism of the groundwater micropollutant 2,6-dichlorobenzamide beyond 2,6-dichlorobenzoate is plasmid encoded in Aminobacter sp. MSH1
| Content Provider | Semantic Scholar |
|---|---|
| Author | Syen, Jeroen T’ Raes, Bart Horemans, Benjamin Tassoni, Raffaella Leroy, Baptiste Lood, Cédric Noort, Vera Van Lavigne, Rob Wattiez, Ruddy Kohler, Hans-Peter E. Springael, Dirk |
| Copyright Year | 2018 |
| Abstract | Aminobacter sp. MSH1 uses the groundwater micropollutant 2,6-dichlorobenzamide (BAM) as sole source of carbon and energy. In the first step, MSH1 converts BAM to 2,6-dichlorobenzoic acid (2,6-DCBA) by means of the BbdA amidase encoded on the IncP-1β plasmid pBAM1. Information about the genes and degradation steps involved in 2,6-DCBA metabolism in MSH1 or any other organism is currently lacking. Here, we show that the genes for 2,6-DCBA degradation in strain MSH1 reside on a second catabolic plasmid in MSH1, designated as pBAM2. The complete sequence of pBAM2 was determined revealing that it is a 53.9 kb repABC family plasmid. The 2,6-DCBA catabolic genes on pBAM2 are organized in two main clusters bordered by IS elements and integrase genes and encode putative functions like Rieske mono-/dioxygenase, meta-cleavage dioxygenase, and reductive dehalogenases. The putative mono-oxygenase encoded by the bbdD gene was shown to convert 2,6-DCBA to 3-hydroxy-2,6-dichlorobenzoate (3-OH-2,6-DCBA). 3-OH-DCBA was degraded by wild-type MSH1 and not by a pBAM2-free MSH1 variant indicating that it is a likely intermediate in the pBAM2-encoded DCBA catabolic pathway. Based on the activity of BbdD and the putative functions of the other catabolic genes on pBAM2, a metabolic pathway for BAM/2,6-DCBA in strain MSH1 was suggested. |
| Starting Page | 7963 |
| Ending Page | 7979 |
| Page Count | 17 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s00253-018-9189-9 |
| Alternate Webpage(s) | https://docnum.umons.ac.be/Access/WebOpenAccess/GetDocument.aspx?Filename=10.1007@s00253-018-9189-9.pdf&GuidTicket=eb6016be-3894-47a6-94b4-1caac718d461 |
| PubMed reference number | 29984394 |
| Alternate Webpage(s) | https://doi.org/10.1007/s00253-018-9189-9 |
| Journal | Medline |
| Volume Number | 102 |
| Journal | Applied Microbiology and Biotechnology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
