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[Simultaneous determination of 14 sexual hormones in health care products by ultra performance liquid chromatography-atmospheric pressure chemical ionization-triple quadrupole mass spectrometry].
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rao, Yakun Li, Zhuo Zhang, Diyue Dong, Manman Wei, Xinghua Chen, D. Zhang, Yafeng |
| Copyright Year | 2019 |
| Abstract | A comprehensive analytical method was developed for the simultaneous determination of 14 sexual hormones in health care products by ultra performance liquid chromatography-atmospheric pressure chemical ionization-triple quadrupole mass spectrometry (UPLC-APCI-MS/MS). The samples were extracted twice with acetonitrile and the extracts were purified using hydrophilic-lipophilic balance (HLB) cartridges. Chromatographic separation was achieved on a Hypersil Gold C18 column (100 mm×2.1 mm, 1.9 μm) by gradient elution with acetonitrile and 10 mmol/L ammonium acetate aqueous solution as the mobile phases. Compounds were detected by APCI-MS/MS with external standard calibration curve method. All the 14 sexual hormones showed a good linear trend in their respective concentration ranges and the correlation coefficients (r) were no less than 0.996. The limits of detection (LODs) ranged from 0.0990 μg/kg to 2.09 μg/kg. The limits of quantification (LOQs) were in the range of 0.495-5.23 μg/kg. The average recoveries of the 14 sexual hormones in the health care product samples ranged from 65.8% to 118.8% at the three spiked levels. The precisions (n=6) ranged from 0.6% to 8.7% (n=6). The method is simple, sensitive, accurate and precise, and is suitable for the determination of the illegal added sexual hormones in the health care products. |
| Starting Page | 626 |
| Ending Page | 633 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.3724/SP.J.1123.2018.12039 |
| PubMed reference number | 31152513 |
| Journal | Medline |
| Volume Number | 37 |
| Issue Number | 6 |
| Alternate Webpage(s) | http://www.chrom-china.com/fileup/PDF/sp1812039.pdf |
| Alternate Webpage(s) | https://doi.org/10.3724/SP.J.1123.2018.12039 |
| Journal | Se pu = Chinese journal of chromatography |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
