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Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Liu, Tianbao Li, Zhen Shen, Yuning Zhu, Hailiang Qian, Yong Ming |
| Copyright Year | 2015 |
| Abstract | We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R(2) = 0.9867) versus hydrogen sulfide concentration in the range of 1-12 μM was obtained. This probe exhibited a remarkable fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm). This probe was successfully utilized to monitor H2S under in vitro physiological conditions and for imaging H2S in living cells and living zebrafish in vivo. |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/c5an00119f |
| Alternate Webpage(s) | http://pubs.rsc.org/en/content/getauthorversionpdf/C5AN00119F |
| PubMed reference number | 26401525 |
| Alternate Webpage(s) | https://doi.org/10.1039/c5an00119f |
| Journal | Medline |
| Volume Number | 140 |
| Issue Number | 21 |
| Journal | The Analyst |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |