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Pythium Seed Rot and Damping-off Resistance
| Content Provider | Semantic Scholar |
|---|---|
| Author | Altier, Nora Barnes, David K. A. Thies, Judy A. Samac, Deborah A. |
| Copyright Year | 1995 |
| Abstract | Source..................... Baiting with alfalfa seedlings in infested soil. (See sources of inoculum section). Storage/Temp ......... Isolates stored on cornmeal agar (4 C) will remain viable for 4-6 months. Isolates stored in sterile water (room temperature) will remain viable for at least 12 months. Production .............. Cornmeal agar inoculated with an isolate from stored cultures and incubated at 24 C for 2-3 days. Preparation.............. A 3 mm-diameter disc of inoculum is removed from the periphery of the 2-3 day-old colony growing on cornmeal agar and placed in the center of a separate 9 cm-diameter petri plate containing 1.5% water agar. Plates are incubated 3 days at 24 C prior to plating seeds. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.naaic.org/stdtests/pythium.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |