NDLI: 60 Hz magnetic field exposure inhibits protein Kinase C dependent induction of Neuropeptide Y mRNA in PC-12 cells

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60 Hz magnetic field exposure inhibits protein Kinase C dependent induction of Neuropeptide Y mRNA in PC-12 cells

Content Provider	Semantic Scholar
Author	Thomas, William J.
Copyright Year	1994
Abstract	Neuropeptide Y(NPY),discovered in 1982, is one ofthe most abimdant neuropeptides found in the mammalian brain. It is also stored and secreted as a hormone from chromaffm cells in the body. It has been shown to regulate cardiac blood pressure, intracellular calcium concentrations in tissue culture, and pineal gland production and secretion of the hormonemelatonin, all which have been shown to be affected by exposure to magnetic fields(MF). In this thesis,I examined the effects of MF exposure on the chemically induced production ofNPY mRNA in the rat pheochromocytoma,PC-12cell line. NPY mRNA production is regulated in these cells by at least three differerii signal transduction mechanisms,the phosphoinositide/protein kinase C(PRC)dependent, the cAMP dependent, and a tyrosine kinase dependent mechanism with a synergism occurring upon simultaneous stimulation of both the phosphoinositide/PKC and the cAMP dependent pathways. There is a significant decline in the synergistic production ofNPY mRNA in PC-12 Cells stimulated with forskolin(20uM)and TPA(50nM)upon magnetic field exposure. This decline was seen at 8 and 24 hours ofchemical plus MF exposure. To determine whether or not a specific signal transduCtiOn pathway wks affected by MF exposure,PC-12 cells were exposed to TPA(12-0-tetradecanoylphorbol-13-acetate) (50nM),or forskolin(20uM)for 8 hows with and without MF exposure. No changes in NPY mRNA were seen between magnetic field and sham exposed unstimulated cells, or in those cells exposed to forskolin(20uM). In three offow experiments, those cells exposed to TPA(50nM)and magnetic field showed an average 27%,36%,
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://scholarworks.lib.csusb.edu/cgi/viewcontent.cgi?article=1907&context=etd-project
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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