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Metabolism and excretion of oestradiol-17 b and progesterone in the Sumatran rhinoceros ž / Dicerorhinus sumatrensis
| Content Provider | Semantic Scholar |
|---|---|
| Author | Heistermann, Michael Agil, Mangestuti Buthe, Andreas Hodges, John Keith |
| Copyright Year | 1998 |
| Abstract | H-labelled oestradiol-17b and C-progesterone were injected i.v. into an adult female Ž . Sumatran rhinoceros Dicerorhinus sumatrensis and all urine and faeces collected over 4 days. Of the injected steroid, 68% of H-oestradiol and 89% of C-progesterone were recovered. Peak excretion in urine occurred on day 1 for both steroids, and for faeces on day 2 for C-progesterone, and between days 2 and 3 for H-oestradiol. Oestradiol metabolites were predominantly Ž . nearly 70% excreted into the urine, while progesterone metabolites were almost exclusively Ž . Ž . )99% excreted into the faeces. The majority )70% of urinary excreted oestrogens consisted Ž . of water-soluble i.e., conjugated forms, with )90% of these being glucuronides. In contrast, Ž )75% of faecal oestrogen and progesterone metabolites were excreted as ether-soluble i.e., . unconjugated forms. HPLC co-chromatography of oestrogens in hydrolysed urine indicated only one peak of radioactivity, co-eluting with authentic oestradiol-17b, whereas two peaks of radioactivity were found after HPLC of faecal oestrogens, the major one co-eluting with oestrone and the less prominent one with oestradiol-17b. Progesterone was excreted as numerous metabolites into the faeces. The three most abundant of these were identified using HPLC and gas Ž . chromatography mass spectrometry GCMS as 5b-pregnane-3a ,20a-diol, 5b-pregnane-3a-ol-20one, and a second pregnanediol, the exact structure of which could not be deduced. Measurement of urinary oestradiol-17b and faecal immunoreactive pregnanediol and 5a-pregnane-3a-ol-20-one ) Corresponding author. Tel.: q49 551 3851141; fax: q49 551 3851288; e-mail: mheiste@gwdg.de 0378-4320r98r$ see front matter q 1998 Elsevier Science B.V. All rights reserved. Ž . PII: S0378-4320 98 00111-0 ( ) M. Heistermann et al.rAnimal Reproduction Science 53 1998 157–172 158 in daily samples enabled the first endocrine characterization of the ovarian cycle and indicated a cycle length of ;25 days. q 1998 Elsevier Science B.V. All rights reserved. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.rhinoresourcecenter.com/pdf_files/117/1175855997.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
