Chromosomal instability in MYH- and APC-mutant adenomatous polyps.

Content Provider	Semantic Scholar
Author	Cardoso, Joana Darc Molenaar, Lia Menezes, Renée X. De Leerdam, Monique Esther Van Rosenberg, Carla Moeslein, Gabriela Sampson, Julian R. Morreau, Hans Boer, Judith M. Fodde, Riccardo
Copyright Year	2006
Abstract	The vast majority of colorectal cancers display genetic instability, either in the chromosomal instability (CIN) or microsatellite instability (MIN) forms. Although CIN tumors are per definition aneuploid, MIN colorectal cancers, caused by loss of mismatch repair function, are usually near diploid. Recently, biallelic germ line mutations in the MYH gene were found to be responsible for MYH-associated polyposis (MAP), an autosomal recessive predisposition to multiple colorectal polyps, often indistinguishable from the dominant familial adenomatous polyposis (FAP) syndrome caused by inherited APC mutations. Here, we analyzed MYH- and APC-mutant polyps by combining laser capture microdissection, isothermal genomic DNA amplification, and array comparative genomic hybridization. Smoothed quantile regression methods were applied to the MAP and FAP genomic profiles to discriminate chromosomes predominantly affected by gains and losses. Up to 80% and 60% of the MAP and FAP polyps showed aneuploid changes, respectively. Both MAP and FAP adenomas were characterized by frequent losses at chromosome 1p, 17, 19, and 22 and gains affecting chromosomes 7 and 13. The aneuploid changes detected at early stages of MYH-driven tumorigenesis may underlie accelerated tumor progression, increased cancer risk, and poor prognosis in MAP.
Starting Page	37
Ending Page	41
Page Count	5
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://cancerres.aacrjournals.org/content/canres/66/5/2514.full.pdf
Alternate Webpage(s)	http://cancerres.aacrjournals.org/content/canres/66/5/2514.full.pdf?origin=publication_detail
PubMed reference number	16510566v1
Volume Number	66
Issue Number	5
Journal	Cancer research
Language	English
Access Restriction	Open
Subject Keyword	Adenomatous Polyposis Coli Adenomatous Polyps Aneuploidy Autosomal recessive inheritance Carcinogenesis Chromosomal Instability Chromosomes Colorectal Neoplasms Colorectal polyposis Diploidy Hepatocellular Adenoma Laser Capture Microdissection MUTYH gene Mismatch Repair Mutation Nucleic Acid Hybridization Pituitary Neoplasms Short Tandem Repeat Specimen Source Codes - Polyps Tumor Progression dna amplification
Content Type	Text
Resource Type	Article