NDLI: Effectiveness of enterobacterial repetitive intergenic consensus PCR and random amplified polymorphic DNA fingerprinting for Helicobacter pylori strain differentiation.

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Effectiveness of enterobacterial repetitive intergenic consensus PCR and random amplified polymorphic DNA fingerprinting for Helicobacter pylori strain differentiation.

Content Provider	Semantic Scholar
Author	Finger, S. Alison Velapatiño, Billie Kosek, Margaret N. Santivañez, Livia Dailidiene, Daiva Quino, Willi Balqui, Jacqueline Herrera, P. Juan Manuel Berg, Douglas E. Gilman, Robert H.
Copyright Year	2006
Abstract	We compared the robustness and discriminatory power of the enterobacterial repetitive intergenic consensus (ERIC) and random amplified polymorphic DNA (RAPD) fingerprinting methods for detecting cases of mixed Helicobacter pylori infection in Peruvian shantytown residents. H. pylori isolates from 63 participants were cultured, and five single colonies and a pool of additional colonies from each participant were analyzed by ERIC-PCR and by RAPD tests with four 10-nucleotide primers (one primer per reaction). There was 94% agreement between the ERIC and RAPD profiles in classifying sets of isolates as uniform versus closely related but not identical versus probably unrelated, indicating a high kappa statistic of 0.8942. Subtle differences in related ERIC or RAPD patterns likely reflect gene transfer between strains, recombination, and/or mutation, whereas markedly different patterns reflect infection by unrelated strains. At least half of infected shantytown residents seemed to carry more than one H. pylori strain, although in 19 of 31 persons, the strains were closely related. Three RAPD tests, each with a different primer, were needed to achieve the sensitivity of one ERIC test. ERIC-PCR constitutes a resource- and time-efficient method for H. pylori strain differentiation.
File Format	PDF HTM / HTML
DOI	10.1128/AEM.00894-06
PubMed reference number	16820463
Journal	Medline
Volume Number	72
Issue Number	7
Alternate Webpage(s)	http://digitalcommons.wustl.edu/cgi/viewcontent.cgi?article=3029&context=open_access_pubs
Alternate Webpage(s)	https://digitalcommons.wustl.edu/cgi/viewcontent.cgi?article=3029&context=open_access_pubs&httpsredir=1&referer=
Alternate Webpage(s)	https://doi.org/10.1128/AEM.00894-06
Journal	Applied and environmental microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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