Comparative effects of FK-506, rapamycin and cyclosporin A, on the in vitro differentiation of dorsal root ganglia explants and septal cholinergic neurons

Content Provider	Semantic Scholar
Author	Carreau, A. Gueugnon, J. F. Benavides, Jesús Vigé, Xavier
Copyright Year	1997
Abstract	There is increasing evidence that immunophilins play a role in neural development and differentiation. We have studied the neurotrophic effects of FK-506, rapamycin and cyclosporin A (CsA) on dorsal root ganglia (DRG) taken from different segmental levels (cervical, thoracic and lumbar/sacral), and on rat embryonic septal cholinergic neurons in culture. At a low concentration (1 nM), FK-506 significantly increased (+83%) the number of neurites of thoracic DRG explants. At a higher concentration (100 nM), it also enhanced the neuritogenesis of thoracic (+100%) and lumbar/sacral (+57%) DRG, but not cervical DRG explants. Rapamycin displayed a converse effect, reducing the development of DRG explants from cervical and thoracic segments (-78% at 1 nM in thoracic DRG). CsA (from 1 to 100 nM) was without effect on DRG neuritogenesis. In contrast to nerve growth factor (NGF), which increased neurite length (+116% at 3 ng/ml), neither FK-506 nor rapamycin affected this parameter.
Starting Page	1755
Ending Page	1762
Page Count	8
File Format	PDF HTM / HTML
DOI	10.1016/S0028-3908(97)00160-3
PubMed reference number	9517448
Journal	Medline
Volume Number	36
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S0028390897001603
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S0028390897001603?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/S0028-3908%2897%2900160-3
Journal	Neuropharmacology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article