NDLI: Expression of 92-kD type IV collagenase/gelatinase (gelatinase B) in osteoarthritic cartilage and its induction in normal human articular cartilage by interleukin 1.

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Expression of 92-kD type IV collagenase/gelatinase (gelatinase B) in osteoarthritic cartilage and its induction in normal human articular cartilage by interleukin 1.

Content Provider	Semantic Scholar
Author	Mohtai, Masaaki Smith, Robert L. Schurman, David J. Tsuji, Yoshiyuki Torti, Frank M. Hutchinson, N. I. Stetler-Stevenson, William G. Goldberg, Gregory I.
Copyright Year	1993
Abstract	We report here that a 92-kD gelatinolytic metalloproteinase is expressed as protein and mRNA in human osteoarthritic cartilage, but not in normal adult articular cartilage. Western immunoblotting demonstrated that the 92-kD gelatinolytic activity corresponded to 92-kD type IV collagenase/gelatinase (gelatinase B); mRNA for gelatinase B was identified by Northern blotting. Chondrocytes from normal cartilage also exhibited mRNA for 72-kD type IV collagenase/gelatinase (gelatinase A), tissue collagenase, and stromelysin-1, and these mRNAs were increased in osteoarthritic cartilage. Regional analysis of osteoarthritic cartilage samples from four individuals revealed that gelatinase B mRNA was expressed in grossly fibrillated areas; two of four nonfibrillated cartilage samples failed to exhibit the mRNA, but did have increased levels of mRNA for other neutral metalloproteinases. IL-1 alpha treatment of normal human cartilage explants or isolated chondrocytes induced increased levels of gelatinase B and increased mRNA for tissue collagenase and stromelysin-1. Under identical conditions, mRNA levels for gelatinase A were not increased indicating that regulation of this enzyme in human articular chondrocytes is distinct from that of other metalloproteinases. Our data showing expression of gelatinase B in fibrillated cartilage suggest that it is a marker of progressive articular cartilage degradation in osteoarthritis.
File Format	PDF HTM / HTML
DOI	10.1172/JCI116547
Alternate Webpage(s)	http://dm5migu4zj3pb.cloudfront.net/manuscripts/116000/116547/JCI93116547.pdf
PubMed reference number	8325982
Alternate Webpage(s)	https://doi.org/10.1172/JCI116547
Journal	Medline
Volume Number	92
Issue Number	1
Journal	The Journal of clinical investigation
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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