NDLI: Mechanism of DNA binding enhancement by hepatitis B virus protein pX.

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Mechanism of DNA binding enhancement by hepatitis B virus protein pX.

Content Provider	Semantic Scholar
Author	Palmer, Christopher R. Gegnas, Laura D. Schepartz, Alanna
Copyright Year	1997
Abstract	At least three hundred million people worldwide are infected with the hepatitis B virus (HBV), and epidemiological studies show a clear correlation between chronic HBV infection and the development of hepatocellular carcinoma. HBV encodes a protein, pX, which abducts the cellular transcriptional machinery in several ways including direct interactions with bZIP transcription factors. These interactions increase the DNA affinities of target bZIP proteins in a DNA sequence-dependent manner. Here we use a series of bZIP peptide models to explore the mechanism by which pX interacts with bZIP proteins. Our results suggest that pX increases bZIP.DNA stability by increasing the stability of the bZIP dimer as well as the affinity of the dimer for DNA. Additional experiments provide evidence for a mechanism in which pX recognizes the composite structure of the peptide.DNA complex, not simply the primary peptide sequence. These experiments provide a framework for understanding how pX alters the patterns of transcription within the nucleus. The similarities between the mechanism proposed for pX and the mechanism previously proposed for the human T-cell leukemia virus protein Tax are discussed.
Starting Page	17
Ending Page	17
Page Count	1
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://schepartzlab.yale.edu/research/Palmer1997.pdf
PubMed reference number	9398264v1
Volume Number	36
Issue Number	49
Journal	Biochemistry
Language	English
Access Restriction	Open
Subject Keyword	CELF Proteins Cell Nucleus Cellular Phone Epidemiology Hepatitis B Virus Hepatitis C virus Herpesvirus 1, Cercopithecine Leukemia, B-Cell Leukemia, T-Cell Liver carcinoma Nesiritide Staphylococcal Protein A TRANSCRIPTION FACTOR Transcription, Genetic Viral Proteins dna binding leukemia
Content Type	Text
Resource Type	Article

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