NDLI: Potentiation of NMDA currents by pituitary adenylate cyclase activating polypeptide in neonatal rat sympathetic preganglionic neurons.

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Potentiation of NMDA currents by pituitary adenylate cyclase activating polypeptide in neonatal rat sympathetic preganglionic neurons.

Content Provider	Semantic Scholar
Copyright Year	1997
Abstract	Whole cell patch-clamp recordings were made from sympathetic preganglionic neurons (SPNs) in the intermediolateral cell column of thoracolumbar spinal cord slices of 12- to 16-day-old rats, and the effects of pituitary adenylate cyclase activating polypeptide (PACAP)-38 on N-methyl-D-aspartate (NMDA)- and kainate (KA)-induced inward currents were examined. PACAP, in concentrations (10-30 nM) that caused no significant change of holding currents, reversibly increased NMDA-induced currents but not KA-induced currents. At higher concentrations (>30 nM), the peptide produced a sustained inward current. The potentiating effect of PACAP was nullified by prior incubation of the slices with the adenylate cyclase inhibitor MDL-12,330A (25 microM). Further, superfusing the slices with the membrane-permeable cyclic AMP analogue N6,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate (100-300 microM) in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (700 microM) increased the NMDA currents. This result suggests that PACAP selectively increases NMDA-receptor-mediated responses in the rat SPNs, probably via a cyclic-AMP-dependent mechanism, providing evidence that the peptide may be involved in synaptic plasticity.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jn.physiology.org/content/jn/78/2/1175.full.pdf
PubMed reference number	9307147v1
Volume Number	78
Issue Number	2
Journal	Journal of neurophysiology
Language	English
Access Restriction	Open
Subject Keyword	1-Methyl-3-isobutylxanthine Adenosine Monophosphate Adenylate Cyclase Adenylyl Cyclase Inhibitors Analog Aspartic Acid Cyclic AMP Cyclic GMP Kainate MDL 12330A N-Methylaspartate Neuronal Plasticity Phosphodiesterase Inhibitors Polypeptides Posterior Pituitary Hormones Spinal Cord Lateral Horn Tissue membrane chemosensitization/potentiation nullified - ManagedParticipationStatus
Content Type	Text
Resource Type	Article

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