NDLI: Concentration of the baculovirus Autographa californica M nucleopolyhedrovirus (AcMNPV) by ultrafiltration

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Concentration of the baculovirus Autographa californica M nucleopolyhedrovirus (AcMNPV) by ultrafiltration

Content Provider	Semantic Scholar
Author	Michalsky, Ronald Passarelli, A. Lorena Pfromm, Peter Czermak, Peter
Copyright Year	2010
Abstract	Abstract Concentration and retention of a rod-shaped virus during tangential flow ultrafiltration (UF) was assessed to evaluate the potential of membrane-based downstream methods with advantages such as easy scale-up for industrial processes. A recombinant baculovirus of the non-spherical Autographa californica M nucleopolyhedrovirus (AcMNPV), vHSGFP, expressing egfp was filtered using polyethersulfone membranes ranging from 30 to 1000 kDa molecular weight cut-off (MWCO). A 20-fold virus concentration was achieved when a membrane cut-off range of 100 to 1000 kDa was tested. Fouling was observed and cake formation and pore plugging were postulated as concurrent causes with different impact depending on the MWCO. A reduction of virus concentration in the range of 2 to 5 log units in the permeate was observed illustrating the potential of membrane-based virus filtration as a useful unit operation in downstream processing.
Starting Page	1125
Ending Page	1127
Page Count	3
File Format	PDF HTM / HTML
DOI	10.1016/j.desal.2009.09.123
Volume Number	250
Alternate Webpage(s)	http://krex.k-state.edu/dspace/bitstream/handle/2097/3523/MichalskyDesalination2010.pdf?isAllowed=y&sequence=1
Alternate Webpage(s)	https://doi.org/10.1016/j.desal.2009.09.123
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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