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Differential EcSmad2 expression in early development of the direct developing frog Eleutherodactylus coqui
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chatterjee, Suman Elinson, Richard P. |
| Copyright Year | 2011 |
| Abstract | crest and their expression is dependent on Wnt, FGF and BMP signaling. The pax3/7 and zic genes have undergone multiple duplications in the evolution of teleost fish. While many pax3/7 and zic paralogs are expressed during neural crest development, their expression is initiated at different times and is seen in different areas along the anterior-posterior axis. These expression differences could result from the gain or loss of regulatory elements in particular pax3/ 7 and zic genes or from modification of existing elements. We identified multiple paralogous enhancer families for the pax3/7 and zic genes and characterized their activity using transgenic zebrafish. The differences in activity of the enhancer paralogs mirror the differences in expression pattern among the genes they regulate. This indicates that the divergent expression patterns of genes in these families are partly the result of enhancer modification. To determine the nature of this enhancer paralog divergence we used small molecule inhibitors and heat shock-induced gene expression to knock down or upregulate components of the Wnt, FGF and BMP pathways in zebrafish lines containing reporter constructs with the enhancers. We found that pax3/7 and zic regulation involves the integration of multiple signals through multiple enhancers. The differences in activity among paralogous enhancers are partly the result of differences in response to these signaling pathways. |
| Starting Page | 247 |
| Ending Page | 247 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.ydbio.2011.05.548 |
| Volume Number | 356 |
| Alternate Webpage(s) | https://core.ac.uk/download/pdf/82532834.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.ydbio.2011.05.548 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
