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Second-harmonic illumination to enhance multispectral digital lensless holographic microscopy.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mendoza-Yero, Omel Carbonell-Leal, Miguel Lancis, Jesus S. Garcia-Sucerquia, Jorge |
| Copyright Year | 2016 |
| Abstract | Multispectral digital lensless holographic microscopy (MDLHM) operating with second-harmonic illumination is shown. Added to the improvement of the spatial resolution of the previously reported MDLHM operating with near-infrared illumination, this second-harmonic MDLHM shows promise as a tool to study the behavior of biological samples under a broad spectral illumination. This illumination is generated by focusing a highly spatially coherent ultrashort pulsed radiation into an uncoated Type 1 β-BaB2O4 (BBO) nonlinear crystal. The second-harmonic MDLHM allows achieving multispectral images of biological samples with enhanced micrometer spatial resolution. The illumination wavelength of the second-harmonic MDLHM can be tuned by displacing a focusing optics with respect to a pinhole; spatially resolved information at different wavelengths of the sample can then be retrieved. |
| File Format | PDF HTM / HTML |
| DOI | 10.1364/OL.41.001062 |
| PubMed reference number | 26974116 |
| Journal | Medline |
| Volume Number | 41 |
| Issue Number | 5 |
| Alternate Webpage(s) | http://repositori.uji.es/xmlui/bitstream/handle/10234/154085/71611.pdf?sequence=1 |
| Alternate Webpage(s) | https://doi.org/10.1364/OL.41.001062 |
| Journal | Optics letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
