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QM/MM study of the inactivation of B12-dependent dehydratases by substrate glycerol.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kovačević, Borislav Barić, Danijela Sandala, Gregory M. Smith, David Matthew Radom, Leo |
| Copyright Year | 2012 |
| Abstract | This reaction is catalysed by two enzymes, glycerol dehydratase (GDH) and diol dehydratase (DDH).1 Both of these enzymes use coenzyme B12 as an essential cofactor., As with most B12-dependent reactions, the mechanism of action of these two enzymes involves the formation of radical intermediates. It is known that both enzymes undergo mechanism based inactivation by their substrate glycerol, the rate of which being higher for DDH than for the GDH.2 The results of computational study of glycerol dehydration catalysed by GDH and DDH are reported. We present and discuss energy profiles of mechanism of action and inactivation, and explain the mechanism of this unusual suicidal inactivation. |
| Starting Page | 64 |
| Ending Page | 64 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://xgironaseminar.files.wordpress.com/2012/05/kovacevic.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
