Resting potential of excitable neuroblastoma cells in weak magnetic fields

Content Provider	Semantic Scholar
Author	Sonniera, H. Kolomytkinb, O. V. Marinoa, A. A.
Abstract	The mechanism by which static and low-fre5-s exposure immediately followed by a 5-s control period. In each experiment, the field had no discernible quency magnetic fields are transduced into biological effect on the transmembrane current in the vicinity of signals responsible for reported effects on brain electrizero current (−50 mV voltage clamp). The sensitivity cal activity is not yet ascertained. To test the hypothesis of the measuring system was such that we would have that fields can cause a subthreshold change in the restdetected a current corresponding to a change in meming membrane potential of excitable cells, we measured brane potential as small as 38 mV. Consequently, if changes in transmembrane current under voltage clamp produced in SH-SY5Y neuroblastoma cells, using the sensitivity of mammalian cells to magnetic fields is mediated by subthreshold changes in membrane potenpatch-clamp method in the whole-cell configuration. In tial, as in sensory transduction of sound, light, and separate experiments, cells were exposed to static fields of 1, 5, and 75 G, to time-varying fields of 1 and 5 G, other stimuli, then the ion channels responsible for the and to combined static and time-varying fields tuned for putative changes are probably present only in specialresonance of Na, K, Ca, or H. To increase ized sensory neurons or neuroepithelial cells. A change sensitivity, measurements were made on cells connected in transmembrane potential in response to magnetic fields is not a general property of excitable cells in by gap junctions. For each cell, the effect of the field was evaluated on the basis of 100 trials consisting of a culture.
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