Loading...
Please wait, while we are loading the content...
Similar Documents
Molecular identification of Lavendula dentata L., Mentha longifolia (L.) Huds. and Mentha × piperita L. by DNA barcodes
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ahmed, Shawkat M. |
| Copyright Year | 2018 |
| Abstract | Five DNA barcodes were tested for identification and discrimination of Lavendula dentata L., Mentha longifolia (L.) Huds. and Mentha × piperita L. New DNA barcodes have been registered for L. dentata from Taif, Saudi Arabia. The separate clading of L. dentata and M. longifolia through the phylogenic analyses proved their endemism to Saudi Arabia. The phylogenetic trees revealed from the ITS2, matK and trnH data demonstrated that all Mentha species formed monophyletic clusters except hybrid M. × piperita from Taif which formed separate clades distinguishing it from the two parents; M. aquatica L. and M. spicata L. DNA barcoding could be considered as a good approach for distinguishing and identifying the mint plants, though it was not possible to confirm the relationship between hybrids and their putative parents. Introduction The family Lamiaceae comprising about 7,173 species under 236 genera possesses medicinal and aromatic herbs such as lavender, basil, mint, rosemary and thyme, that have been widely utilized as teas, spices, traditional medicines or raw material for the food and pharmaceutical industries (Theodoridis et al., 2012). Lavendula dentata is one of five naturally growing lavender species in Saudi Arabia that has been known as the main center of origin of the genus (Miller, 1985). Lavender species as medicinal plants, are distributed in highlands of Albaha, Asir, and Taif and are exploited for the production of high-quality lavender honeys. Locally known as Habak, Al-Madinah mint or wild mint, Mentha longifolia and peppermint, Mentha × piperita are present in the spontaneous flora of Saudi Arabia but also under cultivation. Traditionally, they have been used as medicinal agents to treat colds, cough, headaches, asthma and digestive disorders. Recent studies proved the antiviral, antimicrobial, antioxidant, anti-inflammatory and anticancer characteristics as therapeutic activities for the extracts derived from Mentha species (Anwar et al., 2017). Hybridization and polyploidy play an important role in the speciation of the members belonging to genus Mentha such as M. × piperita that is considered as a hybrid of the two mints; M. spicata and M. aquatica (Mogosan et al., 2017) making them good targets for molecular studies. Various studies have been performed to identify and classify species of Lamiaceae collected from Saudi Arabia based on anatomical and cytological studies (Abdel Khalik, 2016) and biochemical analyses (Kasem, 2016), however, very little is known about DNA barcoding information. DNA sequences for the species under study will be compared in a database against retrieved sequences of identified individuals from the GenBank. If the query sequence matches with one in the database, this will help in identification, discrimination or gaining a new barcodes for these species (Hajibabaei et al., 2007). Therefore, the objectives of this research include: Present address: Biology Department, Faculty of Science, Ta'if University, Ta'if, 5700, Saudi Arabia. Email: shamahmoh@gmail.com |
| Starting Page | 149 |
| Ending Page | 157 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| DOI | 10.3329/bjpt.v25i2.39519 |
| Volume Number | 25 |
| Alternate Webpage(s) | https://www.banglajol.info/index.php/BJPT/article/download/39519/29728 |
| Alternate Webpage(s) | https://doi.org/10.3329/bjpt.v25i2.39519 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
