NDLI: Detection of Listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay.

Content Provider	Semantic Scholar
Author	Wiedmann, Martin Barany, Francis Batt, Carl A.
Copyright Year	1993
Abstract	A polymerase chain reaction (PCR)-coupled ligase chain reaction (LCR) assay for the specific detection of Listeria monocytogenes (M. Wiedmann, J. Czajka, F. Barany, and C. A. Batt, Appl. Environ. Microbiol. 58:3443-3447, 1992) has been modified for detection of the LCR products with a nonisotopic readout. When a chemiluminescent or a colorimetric substrate for the nonisotopic detection of the LCR products was used, the PCR-coupled LCR gave a sensitivity of 10 CFU of L. monocytogenes. The detection method with the chemiluminescent substrate Lumi-Phos 530 permitted detection of the LCR products in less than 3 h, so that the whole assay can be completed within 10 h.
File Format	PDF HTM / HTML
DOI	10.1128/aem.59.8.2743-2745.1993
Alternate Webpage(s)	http://aem.asm.org/content/59/8/2743.full.pdf
PubMed reference number	8368859
Alternate Webpage(s)	https://doi.org/10.1128/aem.59.8.2743-2745.1993
Journal	Medline
Volume Number	59
Issue Number	8
Journal	Applied and environmental microbiology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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