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The protective effects of Achyranthes bidentata polypeptides against NMDA-induced cell apoptosis in cultured hippocampal neurons through differential modulation of NR2A- and NR2B-containing NMDA receptors
| Content Provider | Semantic Scholar |
|---|---|
| Author | Shen, Hongmei Yuan, Ying Ding, Fei Gu, Xiaosong |
| Copyright Year | 2008 |
| Abstract | Achyranthes bidentata Blume is a commonly prescribed Chinese medicinal herb with a variety of pharmaceutical properties. From its aqueous extract we have separated important constituents, referred to as A. bidentata polypeptides (ABPP). In this study, the neuroprotective effect of ABPP against N-methyl-d-aspartate (NMDA)-induced cell apoptosis was investigated in cultured rat hippocampal neurons. The results of MTT assay, Hoechst/PI double staining and DNA ladder detection indicated that ABPP significantly attenuated, in a concentration-dependent manner, apoptotic cell damage induced by exposure of cultured hippocampal neurons to NMDA (100 μM) for 30 min. The intracellular calcium measurement with fluo-3/AM revealed that ABPP antagonized the excess intracellular calcium triggered by NMDA. Furthermore, in addition to inhibiting the action of NR2B-containing NMDA receptors, ABPP can enhance the function of NR2A-containing NMDA receptors. Our data might suggest that ABPP may also prove to be a potential neuroprotective therapy owing to its differential modulation of NR2A- and NR2B-containing NMDA receptors. |
| Starting Page | 274 |
| Ending Page | 281 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.brainresbull.2008.08.002 |
| PubMed reference number | 18765272 |
| Journal | Medline |
| Volume Number | 77 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0361923008002700 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0361923008002700?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/j.brainresbull.2008.08.002 |
| Journal | Brain Research Bulletin |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
