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Analysis of long pyrimidine polynucleotides in HeLa cell nuclear DNA: absence of polydeoxythymidylate.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Birnboim, H. Chaim Mitchel, R. E. J. Straus, Neil A. |
| Copyright Year | 1973 |
| Abstract | HeLa cell nuclear DNA contains an unexpectedly large amount of long pyrimidine polynucleotides. These sequences were detected in DNA labeled with [(3)H] thymidine by treatment with formic acid-diphenylamine and subsequent analysis by polyacrylamide gel electrophoresis. About 0.5% of the total thymine residues were found in polynucleotides that migrated more slowly than 4S RNA. No polynucleotides of comparable size were detected in Escherichia coli DNA. The pyrimidine polynucleotides contained no pure poly(dT) sequences (less than 0.0015% of the total residues), as judged by thermal chromatography of a complex formed with poly(A). |
| Starting Page | 031915 |
| Ending Page | 031915 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/70/8/2189.full.pdf |
| PubMed reference number | 4525425v1 |
| Volume Number | 70 |
| Issue Number | 8 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Contain (action) Diphenylamine Gel Electrophoresis (lab technique) Hydrogen Leukemia, B-Cell Nuclear deoxyribonucleic acid Poly T Polyacrylamide Gel Electrophoresis Polynucleotides Pyrimidine-nucleoside phosphorylase Pyrimidines RNA Thymidine Thymine Tracer formic acid polyacrylamide gels |
| Content Type | Text |
| Resource Type | Article |
