Direct determination of phospholipid lamellar structure at 0.34-nm resolution.

Content Provider	Semantic Scholar
Author	Dorset, Douglas L. Beckmann, Erich Zemlin, Friedrich
Copyright Year	1990
Abstract	Low-dose, high-resolution electron microscopy combined with conventional direct-phasing methods based on the estimates of triplet-structure invariants are used to determine phase values for all observed electron-diffraction-structure factor magnitudes from epitaxially oriented multilamellar paracrystals of the phosphospholipid 1,2-dihexadecyl-sn-glycerophosphoethanolamine. The reverse Fourier transform of these phase-structure factors is a one-dimensional electrostatic potential map that strongly resembles the electron-density maps calculated from similar x-ray-diffraction data. Determination of the phase values for the electron-diffraction data with structure invariants alone is nearly as successful as the combined use of two separate methods, assigning values to 13 of the 16 reflections--i.e., the electrostatic potential map closely resembles the one calculated with all data.
File Format	PDF HTM / HTML
DOI	10.1073/pnas.87.19.7570
PubMed reference number	2217186
Journal	Medline
Volume Number	87
Issue Number	19
Alternate Webpage(s)	http://www.pnas.org/content/87/19/7570.full.pdf
Alternate Webpage(s)	https://doi.org/10.1073/pnas.87.19.7570
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article