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Simultaneous saccharification and co-fermentation of steam-exploded poplar wood to ethanol with Escherichia coli KO 11
| Content Provider | Semantic Scholar |
|---|---|
| Author | Sheng, Z. W. Wang, Qiao Yi Zheng, Luo Luo Zheng, Xiao Yun Wang, Yue Qiang |
| Copyright Year | 2018 |
| Abstract | Steam explosion pretreatment is a promising method for the preparation of lignocellulosic biomass for biofuels production. Steam explosion was performed to assess the effectiveness of pretreating poplar wood for ethanol production. Maintaining steam pressure at 4 MPa for 10 min, 66.8% of hemicellulose and 5.3% of cellulose were hydrolyzed. The scanning electron microscopy observation, FT-IR spectroscopy analysis and X-ray diffraction analysis showed that hydrolysis of hemicellulose and modification of cellulose and lignin structures improved the accessibility of cellulose to hydrolytic enzymes. Cellulase production by Trichoderma reesei RUT C-30 was initiated with inocula prepared by different methods. It was found that the composition of pre-culture medium and inoculum age have minimal or no impact on cellulase production. Using the steam-exploded poplar wood as feedstock with cellulase loading of 20 FPIU g−1 substrate, ethanol production by gene-engineered Escherichia coli KO11 reached 3.6 g L-1, corresponding to 51.4% of the maximum theoretical yield based on glucan remaining in the input substrate. The ethanol yield was limited by the low solid content of steam-exploded residue, and inhibited by the inhibitors released in the pretreatment process. So the steam explosion process needs to be improved for lower water content and less inhibitors, and detoxification is necessary to achieve a good fermentability of pretreated poplar wood to ethanol. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.bcc.bas.bg/BCC_Volumes/Volume_49_Special_K2_2017/BCC-NC15199-49-SI-K2-14-19.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
