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Implementation of a Fully Automated Microbial Cultivation Platform for Strain and Process Screening.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Janzen, Nils H. Striedner, Gerald Jarmer, Johanna Voigtmann, Martin Abad, Sandra Reinisch, Daniela |
| Copyright Year | 2019 |
| Abstract | Advances in molecular biotechnology have resulted in the generation of numerous potential production strains. Because every strain can be screened under various process conditions, the number of potential cultivations is multiplied. Exploiting this potential without increasing the associated timelines requires a cultivation platform that offers increased throughput and flexibility to perform various bioprocess screening protocols. Currently, there is no commercially available fully automated cultivation platform that can operate multiple microbial fed-batch processes, including at-line sampling, deep freezer off-line sample storage, and complete data handling. To enable scalable high-throughput early-stage microbial bioprocess development, a commercially available microbioreactor system and a laboratory robot are combined to develop a fully automated cultivation platform. By making numerous modifications, as well as supplementation with custom-built hardware and software, fully automated milliliter-scale microbial fed-batch cultivation, sample handling, and data storage are realized. The initial results of cultivations with two different expression systems and three different process conditions are compared using 5 L scale benchmark cultivations, which provide identical rankings of expression systems and process conditions. Thus, fully automated high-throughput cultivation, including automated centralized data storage to significantly accelerate the identification of the optimal expression systems and process conditions, offers the potential for automated early-stage bioprocess development. |
| Starting Page | e1800625 |
| Ending Page | e1800625 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| DOI | 10.1002/biot.201800625 |
| PubMed reference number | 30793511 |
| Journal | Medline |
| Alternate Webpage(s) | https://www.bioxcellence.com/sites/default/files/field_bx_document_file/Janzen_et_al-2019-Biotechnology_Journal.pdf |
| Alternate Webpage(s) | https://doi.org/10.1002/biot.201800625 |
| Journal | Biotechnology journal |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
