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Efeitos de nanoemulsões à base de óleo de açaí (Euterpe oleracea mart.) e ácido anacárdico em células de câncer de mama
| Content Provider | Semantic Scholar |
|---|---|
| Author | Alexandre, Thamara Rodrigues |
| Copyright Year | 2018 |
| Abstract | The neoplasia with the highest incidence among women worldwide is breast cancer. Euterpe oleracea Mart., Commonly known as açaí, has been extensively studied in recent years due to its high polyphenols, which have antiproliferative, pro-apoptotic, tumor suppression and adipogenesis, oxidative stress and inflammation activities. Anacardium occidentale, commonly known as cashew, derives from its chestnut a dark liquid with a viscous characteristic called LCC, in it is a compound called anacardic acid (AA), in which it has been used in several researches due to its antitumor activities, antioxidant, molluscicide, antimicrobials, gastroprotectives and antioxidants. The Brazilian biodiversity presents a great variety of phytochemicals, which like these, are little soluble in aqueous solutions, which presents complications for its administration and also the absorption by the organism. In view of this, the use of nanostructured compounds proves to be a promising alternative to circumvent these variables and potentiate the biological effects. In view of the above, the objective of this present project was to evaluate the therapeutic effects of the treatment associated with açaí oil-based nanoemulsions (AçNE) and free anacardic acid in breast cancer cells with metastatic potential (4T1) in vitro. Stability tests evaluated that the nanoglobes present in açaí oil-based nanoemulsions (AçNE) have a hydrodynamic diameter of ± 170 nm, with polydispersion index of 0.220, surface potential of ± 17.5 mV, pH 7, and stability of its physical-chemical properties for 120 days. To perform the experiments, AçNE based on açaí oil was used and the AçNE with change in its surface were also used, being polymers of chitosan (CH) or polyethylene glycol (PEG). These nanoformulations had a cytotoxic effect on the murine adenocarcinoma 4T1 lineage. However, PEG nanoformulation, regardless of the association to nanoforming with AçNE, showed cytotoxicity and was disregarded for subsequent assays. The nanoemulsions of AçNE and AçNE with CH presented similarity in the reduction of cell viability in tests with the addition of AA, showing no combinatorial effect. However, the combinatorial effect was observed in the use of serial treatment (24-hour interval for each treatment). Although AçNE and AçNE CH still have similar effects, AçNE was chosen because of the more cost-effective and simpler formulation protocol. Data acquired in flow cytometry suggested cell death by apoptosis in serial treatment with AçNE and AA. Results analyzed in the wound healing assay showed the inhibitory and combinatorial effect of AçNE and AA. Thus, the present study suggests that the combinatorial effect between açaí oil-based nanoemulsion and AA may be an alternative for future antineoplastic therapies to be better informed in future studies. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repositorio.unb.br/bitstream/10482/31977/1/2018_ThamaraRodriguesAlexandre.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
