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Spectroscopic Investigations of [FeFe] Hydrogenase Maturated with [(57)Fe2(adt)(CN)2(CO)4](2-).
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gilbert-Wilson, Ryan Siebel, Judith F. Adamska-Venkatesh, Agnieszka Pham, Cindy C. Reijerse, Edward J. Wang, Hongxin Cramer, Stephen P. Lubitz, Wolfgang Rauchfuss, Thomas B. |
| Copyright Year | 2015 |
| Abstract | The preparation and spectroscopic characterization of a CO-inhibited [FeFe] hydrogenase with a selectively (57)Fe-labeled binuclear subsite is described. The precursor [(57)Fe2(adt)(CN)2(CO)4](2-) was synthesized from the (57)Fe metal, S8, CO, (NEt4)CN, NH4Cl, and CH2O. (Et4N)2[(57)Fe2(adt)(CN)2(CO)4] was then used for the maturation of the [FeFe] hydrogenase HydA1 from Chlamydomonas reinhardtii, to yield the enzyme selectively labeled at the [2Fe]H subcluster. Complementary (57)Fe enrichment of the [4Fe-4S]H cluster was realized by reconstitution with (57)FeCl3 and Na2S. The Hox-CO state of [2(57)Fe]H and [4(57)Fe-4S]H HydA1 was characterized by Mössbauer, HYSCORE, ENDOR, and nuclear resonance vibrational spectroscopy. |
| Starting Page | 8998 |
| Ending Page | 9005 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| DOI | 10.1021/jacs.5b03270 |
| PubMed reference number | 26091969 |
| Journal | Medline |
| Volume Number | 137 |
| Issue Number | 28 |
| Alternate Webpage(s) | http://chemgroups.ucdavis.edu/~cramer/Publications_pdf/cramer_233.pdf |
| Alternate Webpage(s) | https://doi.org/10.1021/jacs.5b03270 |
| Journal | Journal of the American Chemical Society |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
