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Erythrocyte glucose-6-phosphate dehydrogenase activity assay and affinity for its substrate under "physiological" conditions.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Leite, A. Z. A. Barretto, Orlando César De Oliveira Pereira |
| Copyright Year | 1998 |
| Abstract | Glucose-6-phosphate dehydrogenase (G6PD) activity and the affinity for its substrate glucose-6-phosphate were investigated under conditions similar to the physiological environment in terms of ionic strength (I: 0.188), cation concentration, pH 7.34, and temperature (37 degrees C). A 12.4, 10.4 and 21.4% decrease was observed in G6PD B, G6PD A+ and G6PD A- activities, respectively. A Km increase of 95.1, 94.4 and 95.4% was observed in G6PD B, G6PD A+ and G6PD A-, respectively, leading to a marked decrease in affinity. In conclusion, the observation of the reduced activity and affinity for its natural substrate reflects the actual pentose pathway rate. It also suggests a much lower NADPH generation, which is crucial mostly in G6PD-deficient individuals, whose NADPH availability is poor. |
| Starting Page | 51 |
| Ending Page | 59 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.scielo.br/pdf/bjmbr/v31n12/3208c.pdf |
| PubMed reference number | 9951548v1 |
| Volume Number | 31 |
| Issue Number | 12 |
| Journal | Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Affinity Cations Deficiency of glucose-6-phosphate dehydrogenase Erythrocytes Glucose-6-phosphate dehydrogenase deficiency anemia Ionic Strength Pentoses acyl-CoA dehydrogenase glucose-6-phosphate dehydrogenase activity |
| Content Type | Text |
| Resource Type | Article |
